View   
     List   
     FAQ   
  Home   /  Medicine of sensory organs  /  Corneal and Refractive Surgical Unit  
  Config  
 | 
  Log in (Admin)  
    
     
 
   
Corneal and Refractive Surgical Unit
   
Zoltán Z. Nagy, MD, PhD, DSc
    Professor

Our research interests focus on two main fields: neuroprotection in the ischemic brain and investigation of the brain microvessel endothelium as a blood-brain barrier (BBB) model. We are experienced in the culture of different cell types, neuronal cells (PC12), fibroblasts, vascular smooth muscle cells, cells from early restenosis, and endothelial cells especially from human brain microvessel (HBEC). Furthermore transient and permanent brain ischemia models are used to study apoptosis and repair mechanisms of neurons in the ischemic penumbra. Different anti-apoptosis strategies, stem cell implantation, anti-apoptotic bcl genes transfer using adenovirus constructs and propargyl-amine molecules are tested after ischemia or hypoxia. Repair strategies are measured with behavioral tests. Different signal molecules are monitored with immunocyto(hysto)chemical methods by confocal microscope. The protein expressions are detected with Western blot and ELISA. Genetic methods are completed our techniques. In the laboratory, a new double labeling method was developed to simultaneous measure of ROS and mitochondrial permeability pore opening. We described a reverse regulation of HBEC and myointimal hyperplasia cell proliferation by a heat-shock protein co-inducer after hypoxia.  
Our previous endothelial studies demonstrate that the HBEC culture appears to be a relevant system to study the interaction between endothelial cells and the pathological factors (hypoxia, elevated level of inflammatory cytokines, hemostatic factors and lipids). Presently we are characterizing the expression of efflux transporters of the ATP binding-cassette superfamily on HBEC. We are modulating their expression and efflux activity in physiological and pathological circumstances. We are investigated the expression of different genes according to whether are constitutively or inducibly expressed in HBEC culture, and to whether are specific to HBEC.  		   
   
        
   
   
KEYWORD(S): blood-brain barrier, neuroprotection, apoptosis, neuronal plasticity, early restenosis, 
   
  1 - 2 (2)     
 
  1 - 2 (2)     
 
  KEYWORD UNIVERSE: CO2-sensitivity, GTPase activating proteins - GAPs, NADPH oxidase - NOX2, NADPH oxidase, Neutrophilic granulocytes, TASK, TRESK, aging, apoptosis, asthma, autonomic neuropathy, autoregulation, avian, bioinformatics, blood-brain barrier, c, calcineurin, calcium, cancer, cell biology, cell fusion, cerebral blood flow, cerebral blood volume, complement, dendritic cells, diabetes, dopamine, duplikation, early restenosis, embryo, embryomanipulation, endoplasmic reticulum, endothelium, enviroment, epidemiology, exercise, gene expression, gene, genetics, hydrogen peroxide, hypothalamus, immunology, in vitro fertilization, inflammation, ischemia-reperfusion, liver, lupus nephritis, lymphoid tissue, medicinal chemistry, metabolic bone disease, mitochondria, molecular biology, neuronal plasticity, neuroprotection, oral biology, oxidative stress, pathology, pathophysiology, peroxidase, phagocytes, pharmacokinetics, physiology, potassium channel, reactive oxygen species, receptors redox homeostasis, regulation, signal transduction, small GTPases, stem cell, stress, stroke, superoxide, tetrasomi, tissue engineering, transplantation,  
  Corneal and Refractive Surgical Unit  Glaucoma Clinical and Research Group  Molecular and Cellular Neuroendocrine Research Laboratory  Laboratory of Corneal Wound Healing  Cerebrovascular Signaling Research Group  Laboratory of Molecular Genetics  Neuromorphological and Neuroendocrine Research Laboratory  Biochemical Pharmacology Unit  Laboratory of Tumor Biology  Laboratory of Molecular Endocrinology   
     Research Areas     

Behavioural sciences

  

Biochemistry, cell biology, biophysics

  

Dental sciences

  

Experimental and clinical immunology and genetics

  

Experimental and clinical oncology

  

Internal medicine and pediatrics

  

Medicine of sensory organs

  

Miscellaneous

  

Molecular biology, microbiology

  

Morphological sciences (anatomy, pathology, forensic medicine)

  

Neurosciences

  

Pharmaceutical sciences, pharmacology

  

Physiology, pathophysiology

  

Reproductive sciences

  

Sport sciences

  

Surgery (operative sciences)

  
    :: © 2009 - Semmelweis University :: Production - DataWeb Systems ::