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Antibiotic Resistance Research Unit
   
Dóra Szabó, MD, PhD
    Assistant Professor

Our research group has been working on antibiotic resistance mechanisms in Gram-negative bacteria to determinate the molecular basis of beta-lactamase mediated resistance in Gram-negative bacteria. Our group has published the first extended-spectrum – beta-lactamase (ESBL) producing Klebsiella pneumoniae outbreak in Hungary. We investigated the risk factors for colonization and infection of ESBL and non-ESBL-producing K. pneumoniae and K. oxytoca strains. We have also done animal experiments with ESBL-producing K. pneumoniae strain to compare the therapeutic effect of different antibiotics – beta-lactams, aminglycosides, carbapenems and to investigate the in vitro and in vivo inoculum effect. In collaboration with Dr. David Paterson’s laboratory at the University of Pittsburgh, PA, US we have conjointly isolated and identified 5 novel beta-lactamases.  We were working on the ESBL-detection in Enterobacter cloacae too. We developed a real-time PCR method for detection of multiple SHV beta-lactamases in a single isolate. We determinate the antibiotic resistance profile of multidrug-resistance (MDR) Gram-negative bacteria - specially Enterobacteriaceae, Pseudomonas aeruginosa - isolated from clinical samples. We investigate the phenotypic and genotypic backgrounds of the antibiotic resistance special regard to beta-lactams, aminoglycosides and quinolones. We determinate the minimal inhibitory concentrations (MICs) of the tested drugs, the isoelectric points, the kinetic parameters, the presence of the outer membrane proteins and their role in the resistance. We study the genetic background of the resistance mechanisms by detecting with PCR the genes of different beta-lactamases, topoisomerases and other quinolone resistant determinants, the genes of multi-drug efflux pumps. We determinate the sequences of that genes. We study the molecular epidemiological relation among the resistant strains using different molecular techniques. 		   
   
        
   
   
KEYWORD(S): antibiotic resistance, Gram-negative, beta-lactamase, fluoroquinolone, 
   
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  Antibiotic Resistance Research Unit  Ocular Surface and Cornea Laboratory  Clinical Nephrology Research Group  Laboratory of Comparative Neurocytology and Neuroethology  Corneal and Refractive Surgical Unit  Glaucoma Clinical and Research Group  Molecular and Cellular Neuroendocrine Research Laboratory  Laboratory of Corneal Wound Healing  Cerebrovascular Signaling Research Group  Laboratory of Molecular Genetics  Neuromorphological and Neuroendocrine Research Laboratory  Biochemical Pharmacology Unit  Laboratory of Tumor Biology  Laboratory of Molecular Endocrinology   
     Research Areas     

Behavioural sciences

  

Biochemistry, cell biology, biophysics

  

Dental sciences

  

Experimental and clinical immunology and genetics

  

Experimental and clinical oncology

  

Internal medicine and pediatrics

  

Medicine of sensory organs

  

Miscellaneous

  

Molecular biology, microbiology

  

Morphological sciences (anatomy, pathology, forensic medicine)

  

Neurosciences

  

Pharmaceutical sciences, pharmacology

  

Physiology, pathophysiology

  

Reproductive sciences

  

Sport sciences

  

Surgery (operative sciences)

  
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